Meloidogyne enterolobii is considered as the most aggressive root-knot nematodes due mainly for its high reproductive rate, induction of large galls and wide host range. The root knot nematode is not a quarantine pest in Singapore. In 2004, Meloidogyne enterolobii was detected in a cactus sample submitted to the national plant health diagnostic laboratory. The identity of the specimen was verified by overseas expert. Further investigation did not reveal the presence of this nematode on site and the affected plant had been destroyed. in 2010, a survey was initiated to determine the prevalence of this root knot nematode in ornamentals in Singapore. A total of 552 ornamental plants had been collected and checked. Meloidogyne from Ficus sp., Plumeria sp. and Ophiopogon sp. samples were detected but further molecular diagnosis using specific primers revealed that the detected species had DNA base pair of less than 720 indicative of a tropical Meloidogyne species. While there was a range of other plant parasitic nematodes detected in this survey, no Meloidogyne enterolobii was detected at the conclusion of this survey. The detection in 2004 was deemed as an once off incidence. The status of Meloidogyne enterolobii in Singapore is : Absent: No pest records (confirmed by survey).