This report concerns a second update of the official findings of Ralstonia solanacearum, race 1, in the Netherlands at glasshouse companies producing ornamental Rosa plants for planting for cut flower production, reported in September and October 2015. The first finding was triggered following symptoms observed by the grower. The identity of R. solanacearum was confirmed on 11 September 2015, whereby race 1 of R. solanacearum was identified on 23 September 2015. Trace-back and trace-forward investigations have revealed contamination in total at 15 companies in 2015 and 2016, including five propagation companies. The identity of R. solanacearum was confirmed on 22 September 2016 at the last propagation company. Measures have been or will be taken at all companies aimed at prevention of spread and eradication. After eradication and monitoring by taking water samples some of the companies have been declared free from the bacteria. The possible source of the outbreak is still unknown and under investigation.