ELISA tests for plant pathogenic bacteria
Published: Jue, 27 Dic 2012, 16:09Last updated: Lun, 11 May 2015, 16:20
Enzyme Linked Immuno Sorbent Assay (ELISA) tests for bacteria may be used for screening of large numbers of samples as an alternative to immunofluorescence (IF) in certain cases (see PM 7 - 97 Indirect Immunofluorescence test for plant pathogenic bacteria). They can also be used as part of the identification of pure cultures. This standard describes how to perform an ELISA test for detection and - or identification in bacterial diagnostics using: (i) Indirect; (ii) Double antibody sandwich-DAS; (iii) Double antibody sandwich indirect-DASI also named triple antibody sandwich-TAS; and (iv) Direct tissue-print, squash or colony-dot.
Resources on this page are not intended to be interpretations of ISPMs and terminology used may not be consistent with ISPM 5. Unless otherwise indicated resources, or pest distributions contained within them are not endorsed/adopted/agreed by the CPM nor developed under the auspices of the IPPC Secretariat, and are the exclusive responsibility of the author of the contributed phytosanitary resource.
Resource provide by:- EPPO
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Type of contact: ORPF
Tags:
- Diagnostic protocol
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